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. Lectin purification on affinity columns containing reductively aminated disaccharides
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. For each of the amino acid sequences shown in Table 1, ensemble averages and conformalional distributions were obtained from an ensemble with a sample size of 10^sup 4^ and the ensembles were generated as discussed above
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. Experiments at pHAcrylamide quenching experiments, measured by excitation at either 280 or 295 nm, showed exponential Stern-Volmer plots (Table 1 ), which yielded similar results: the K^sub sv^ were larger at acidic than at neutral pH, and the experiments in GdmHCl had the largest values of K^sub sv^
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. This ~90° rotation of the diffusion tensor together with the relatively small structural changes within each CaM domain puts direct restraints on the overall structure of the CaM:SEF2-1mp complex, which allows us to choose one of the two possible schematic structure models proposed in our earlier study (11) (see Figs
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. They can either be in fast exchange or in slow exchange to a lowly populated bound CaM state
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. Processive enzymes, when sliding along their targets (50), have the closest similarity to CaM:SEF2-1mp binding
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. 47 ml g^sup -1^, obtained from the R^sub h^ of the BLG monomer in dilute solution, leads to values of φ for the more concentrated solutions which are above the expected φ^sub max^ or, using the reverse of the above conversion, a mass fraction of 43
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. Interestingly, we found that ANS fluorescence intensity increases as the A-state molten globule of TTR aggregates
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. For example, the CD curve recorded at 1
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. Comparative Biochemistry and Physiology 62A:439-444
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. Proc National Acad Sci 1960:46: 1533-42
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. The angle that a helix axis makes with the main axis of the diffusion tensor can therefore be determined with reasonable accuracy from (τ^sup o^^sub m^)^sub app^ averaged over a helix
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. In CaM:bHLH interactions two flexibly bound complexes form a flexibly bound dimer of complexes, defining a geometric context and thereby increasing the number of interactions and thus leading to high affinity and specificity
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