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. The amide I' bands were assigned according to previous publications (24)
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. Here we report a study of the backbone dynamics by ^sup 15^N-spin relaxation on the CaM dimer in complex with a dimeric peptide that mimics the CaM binding region of the bHLH transcription factor SEF2-1
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. The histogram shows two well-resolved (non-zero) peaks, centered at E ~ 0
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. They also show contacts that are strongly represented in the native state and weak in the denatured state
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. the amide I' band of EI^sup SC^ in the presence of both effectors is shown
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. For HuBChE, it supports the notion that its denaturation is a concerted process, involving a large number of amino acid residues
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.The influence of small differences in primary and quaternary structure and in degree of glycosylationDifferences in primary and quaternary structure, degree of glycosylation, and oligomeric state, between DmAChE and HuBChE, although they affect various structural and functional parameters, are not reflected in changes in global dynamics on an Ångström-nanosecond scale in the temperature range investigated
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.In conclusion, the CaM:SEF2-1mp binding seems indeed distinct from other known protein:target binding modes
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