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For example, on our inpatient side, our health information management group is responsible for compiling charts and getting them to the coders
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. The folding of ACYI has been characterized to be a three-state process involving an inactive dimeric intermediate with molten globule-like characteristics (18,19)
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. Conversely, gSBA showed the same hydrodynamic radii until 3
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. This inherent weakness of the Flory mean-field theory also demands extreme caution when extrapolating simulation or experimental results from peptides (10,12,43,58,101,102,129,138) to draw conclusions about denatured proteins
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. MATERIALS AND METHODS Materials All chemicals were of the highest purity grade available from commercial sources and were used without further purification
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. 1 shows the change in ANS fluorescence induced by the four azurin species upon freezing of the sample at -13°C (200 µM ANS, 2
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. 5 mM KCl, 1 mM CaCl^sub 2^, 1 mM MgCl^sub 2^, 1 mM Na^sub 2^HPO^sub 4^, 5 mM HEPES, pH 7
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. 99%) Cu and Ni of 0
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. Only residues 36, 42, 57, and 115 are exceptions with k^sup -1^^sub ex^ values above ~200 µs
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. To calculate the protein number density, the dimer molecular mass of 36
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. Purified proteins were dissolved in 8 M Urea and refolded by dialysis as described in Gursky and Atkinson (1996)
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. 33 K/min (80 K/h) yields similar CD melting curves (not shown), with the apparent T^sub m^ = 84°C identical to that of intact disks
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. Some characteristic traits of the mature amyloid fibrils are the tendencies to bend, twist, and agglomerate
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. [FIGURES 1-3 OMITTED]Body pigmentation Argentine sandperch larvae were lightly pigmented during all stages of development (Fig
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