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. The changes of thermodynamic parameters (enthalpy change ((Delta)H), entropy change ((Delta)S) and heat capacity change ((delta)C^sub p^)) corresponding to the transitions from N to MG (molten globule state) or MG to U (unfolded state), were summarized in the previous report (Nishii et al
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. Fluorescence spectroscopy Intrinsic fluorescence was measured using a RF-1501 rluorospectrometer (Shimadzu, Duisburg, Germany) and a LS55 luminescence spectrometer (Perkin-Elmer, Rodgau, Germany)
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. As a first step in this direction, we measured the thermal unfolding transition of α-amylases in the presence of NDSB, which is known to be an effective agent to prevent aggregation of thermal unfolded proteins (32-34)
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.33 K/min is observed above 80°C, ~10°C higher than the onset of the corresponding CD transition
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