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It is interesting to compare the efficiencies obtained for iodide quenching of the fluorescence of GR and of free Trp in aqueous solution
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Investigation of the properties of these partially folded states using probes such as ANS or bis-ANS (which bind to exposed hydrophobic domains in proteins) was not possible due to the significant overlap between the emission spectra of these probes when bound to proteins and the excitation spectrum of the prosthetic group, FAD (data not shown)
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2 mg/ml) similar to that used for the activity assay, except that the transitions occurred at a temperature ~2°C higher than the 1 mg/ml sample
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A twofold advantage underlies our choice of sort-core repulsions
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Büldt (Forschungszentrum Jülich) for continuous support in his institute
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Among them LDH, α-amylase, and β-gal have in the past been the subject of freeze-thawing inactivation studies (13-17)
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OBi solder is wettable on Cu and Ni substrates and strongly dependent on the type of flux
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The N-H^sup N^ bond length was set to 1
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A single set of CaM resonances, such as we observe, implies fast exchange and/or slow exchange to a lowly populated state (33,34)
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9), the change in viscoelasticity is a further indication of the onset of a glass transition with increasing BLG concentration
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Thus, the effective size of the monocation in compact DNA is expected to be closer to the size of free ion without hydration shell, where the sequence of the monocation radius is Li+ CONCLUSIONOur investigation highlights the inverse relation between DNA compaction potentials of monovalent cations in a crowded environment and the previously reported DNA binding properties of different alkali ions
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In this study, we determined the volume change for Gβγ-PLCβ interactions in solution and bound to membranes
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Pressures applied should follow the guidelines of the homogenizer manufacturer
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Rationalization of this observation has given rise to a third view of the critical pathway of protein folding, the "topomer search" model (Plaxco and Gross, 2001)
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Many researchers say it should have happened sooner
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They were asked whether they thought the advice had helped them in the past block and whether they thought it would help in future blocks
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