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. Structural correspondence between the α-helix and the random-flight chain resolves how unfolded proteins can have native-like properties
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. Steady-state measurements Spectra were acquired in a 0
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. The peak maxima range from ~308 nm for relatively nonpolar environments inside the protein globule to λ^sub max^-values of 350 nm which represent chromophores at the protein surface surrounded by polar water molecules (5,6)
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. Anilinonaphtalene sulfonate as a probe of membrane composition and function
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. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
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. Figure 4c reveals that 255°C, 275°C, and 295°C temperatures exhibit the maximum wetting force as 2
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. 6 ms and δ = 450 µs is significantly above zero
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. The amount of aggregated material, or the composition of the coexisting phases, was measured by centrifuging the samples for 5 min at 4000 g, removal of the upper phase, and determination of protein content of the aggregates or the concentration in each coexisting phase using spectrophotometry
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. The light-scattering cooling curves recorded at faster rates (1
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. The funneled landscapes then arise through projecting the typically terraced hypergutter structure onto lower dimensional representations
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. Minimum and maximum depths sampled were 20 and 71 m, respectively
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. We note here that although we have interpreted peak shifts and broadening in terms of distance changes and ribozyme subpopulations, respectively, peak shifts could also potentially be caused by solvent effects on dye photophysics, and corresponding changes in the correction factor [gamma] (see Materials and Methods), as well as peak broadening by effects of [kappa]^sup 2^, the orientation factor (Deniz et al
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. "Should We Be Using NSAIDs for the Treatment of Osteoarthritis and "Rheumatism?" [accessed on September 14, 2004]
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. The blood vessels in and around tumors tend to have larger-than-normal pores, so the liposomes pass into and accumulate in cancerous tissues more readily than they do in healthy ones
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. Instead, other factors such as heavier teaching schedules, lack of mentoring or fewer personnel and resources that cut across gender lines make it difficult to single out work habits as a contributing factor in the lack of women's progress in academic pharmacy
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. men did, like the Rotary club, or golf
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- We've been able to implement process changes that have eliminated finger-pointing and barriers, specifically in our coding, dictation, and chargemaster areas
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. Using standards set in 1984, the FDA allows such high levels of cancer-causing PCBs in farmed salmon that, according to an Environmental Working Group study, were they present in wild salmon, the EPA would restrict consumption to one meal per month
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. Iodide is a negatively charged quencher expected to quench the fluorescence emission of solvent-exposed tryptophan residues (Lakowicz, 1983)
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. At basic pHs, the K^sub sv^s were similar, within the error, to those observed at pH 7
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. and 1 1 did not yield a sigmoidal behavior (data not shown)
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. J Immunol 2003; 171:1507-1514(38) Henson PM
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. Structural stability and unfolding properties of thermostable bacterial alpha-amylases: a comparative study of homologous enzymes
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. Developmentally regulated packaging of mitochondrial DNA by the HMG-box protein mtTFA during Xenopus oogenesis
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. MSDs of native and soman-inhibited HuBChE were identical below the thermal denaturation temperature of the native enzyme, indicating a common mean free-energy surface
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. 4) of each sample at a given temperature T was normalized to its elastic intensity at the lowest temperature (~20 K) for each detector, and the values so obtained were plotted in a semilogarithmic plot versus Q^sup 2^
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. If the cytoplasm is dehydrated as, for example, in the desiccation of a seed, the results indicate that when the concentration of globular proteins exceeds 60% w/w this component will vitrify
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.We are grateful to Per Persson for initial assistance with electron microscopy work and to Jeffery Kelly and David Wemmer for providing the pmmHα plasmid
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., 2003a), show that the heat- and denaturant-induced transitions in discoidal lipoproteins are kinetically controlled reactions that involve protein unfolding, dissociation, and lipoprotein fusion
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.RESULTSConfigurational entropy of gp120 and CD4The configurational entropy of various sets of atoms of the free gp120 and CD4 molecules and their complex are shown in Figs
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. The globules in the meteorite comprise layers of mixed (Mg, Fe, and Ca) carbonates, magnetite, and iron sulfides
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.)10Osaka Prefectural FisheriesLate19 May '95Station and Sea FarmingCenter (Osaka Pref
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.23 DNA from calf thymus (Sigma) was used to prepare a standard curve
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.Players' AttitudesTo better understand people's play, we examined the rating data (see Table 4) to determine whether the provision of advice influenced the attitudes of the players toward the game
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