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. These difference maps are shown in Fig
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. For experiments involving PEP, control scans were acquired with PEP added to buffer versus buffer in the reference cell, and baselines were found to be the same as in buffer-buffer baselines, suggesting that under these conditions hydrolysis of PEP during heating was not significant
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. Based on these findings, it is tempting to suggest that the changes monitored by far-UV CD were due to the proximity of residues in the tertiary structure of the whole intact EIC^sup sc^ structure, but not of the isolated domains (and then, in those modeled in this work)
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. This shoulder seems to represent a subpopulation of thermal unfolded states which exhibit less buried tryptophan residues, possibly related to unfolded proteins which are not aggregated
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. For example, the calculated ratio of k^sub cat^ (DmAChE/HuBChE) for hydrolysis of various substrates at 25°C is 1
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. Hydrodynamic calculations on the CaM:SMLCK complex showed that its diffusion tensor is nearly axially symmetric with a (τ^sup o^^sub m^)^sub app^ pattern for the α-helices that is almost a mirror image of that in the CaM:SEF2-1mp complex, Fig
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. This indicates that the intermolecular interactions between gp120 and CD4, although decreasing the intraresidue entropy of residues in direct contact, can induce intraresidue entropy changes at the interdomain interface within the gp120 monomer and at the putative trimeric gp120 interface
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