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. In this case, deglycosylation lowers the T^sub m^ of the peptide by 20°C, whereas the free energy of unfolding is reduced by only 1 kcal/mol (26)
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. Denatured states figure prominently in a variety of studies on proteins especially as reference states for estimating protein stability (4,5,13-18)
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. The wave numbers used in the calculations range from q = 0 to q = 0
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. In particular denatured states of larger multidomain proteins quite often show up as more expanded structures with a strong tendency to form aggregates (1,2)
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. The results clearly indicate that the extent of azurin denaturation-aggregation is correlated to the value of n increasing from WT to mutant proteins and that among the latter species it is significantly larger with the cavity-forming mutants I7S and F110S relative to compact C3A/C26A
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. Protein dynamics: comparison of simulations with inelastic neutron scattering experiments
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. Given the anisotropy and the maximum (τ^sup o^^sub m^), τ^sup o^^sub 1^, the angle Φ that each N-H^sup N^ bond vector makes with the main axis of the diffusion tensor can be calculated
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. It is however notable that the ThT molecule also responds to early oligomers (Fig
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. Although the fluorescence lifetime of ANS is not in the range to enable accurate determination of the rotational correlation time of a large oligomer, ANS anisotropy decay can be used as a measure of aggregation both through point measurements and in a kinetic assay showing limiting anisotropy baselines
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