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the fluorescence was significantly affected by the appearance of serious aggregation
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Biophysical Characterization of the Enzyme I of the Streptomyces coelicolor Phosphoenolpyruvate:Sugar Phosphotransferase SystemHurtado-Gómez, EstefaníaABSTRACT The first protein in the bacterial phosphoenolpyruvate (PEP):sugar phosphotransferase system is the homodimeric 60-kDa enzyme I (EI), which autophosphorylates in the presence of PEP and Mg^sup 2+^
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A Philips CM200 transmission electron microscope operating at 120 kV was used for obtaining micrographs at 100,000-fold × magnification
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At subsaturation levels of membranes, Gβγ dissociates above 1000 atm, which may be due to the phase transition, but at higher lipid concentrations, CPM-Gβγ membrane association is stable throughout the pressure range (Fig
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, light scattering) or selectively detecting the amyloid fibrils (fluorescence of amyloids labeled with thioflavin T; e
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