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Thus the sample with a concentration of 1 mg/ml was used for further analysis
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Fluorescence measurements Fluorescence spectra were collected in a Gary Eclipse spectrolluorometer (Varian, San Carlos, CA) interfaced with a Pettier cell
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The profiles of Fig
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To contribute to the ongoing discussion and to investigate the relationship among molecular dynamics, activity, inhibitor-binding, and unfolding, we carried out incoherent elastic neutron scattering experiments on two members of the cholinesterase (ChE) family: Tetrameric human butyrylcholinesterase (HuBChE), both native and covalently inhibited by the organophosphate nerve agent, soman (18), and dimeric native Drosophila melanogaster acetylcholinesterase (DmAChE)
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Multiple glassy states in a simple model system
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DISCUSSIONIn this study, we have applied high pressure techniques to determine the volume change associated with PLCβ-Gβγ interactions on membrane surfaces and in buffer
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The monomers are prone to fast oligomerization
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