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The above results from IR and intrinsic fluorescence spectroscopy strongly suggested that the thermal unfolding of ACYI was a step-wise process
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Phenylalanine side chain behavior of the intestinal fatty acid-binding protein: the effect of urea on backbone and side chain stability
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Experiments were carried out at pH 4, 7, and 10
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In Advances in Science and Technology, Ser
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For a moderate system size such as the entire CD4-D1 domain with 1021 atoms, convergence of conformational entropy of both the free and bound states is only observed in the later stage of the 8-ns trajectory segments
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Our results illustrate how these secondary structure elements combine to direct the tertiary folding pathway of the ribozyme
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. The reason that the autopeak at 1629 cm^sup -1^ did not appear might have been due to the overlapping of the adjacent intense peak at 1638 cm^sup -1^
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. In high concentrations of denaturants, proteins behave like polymers in a good solvent and ensembles for denatured proteins can be modeled by ignoring all interactions except excluded volume (EV) effects
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. On the other hand, the binding of Mg^sup 2+^ did not alter the structure of the protein
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. However, upon unfolding transitions generally distinct shifts of the emission spectra are reported (see for example (4,9-14))
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. This loss is compensated via multimerization
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. Mg^sup 2+^-dependent folding of a large ribozyme without kinetic traps
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