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. Thus, both the appearance of tyrosine emission and the red-shift of tryptophan fluorescence indicated extensive unfolding of GR at 3 M GdnHCl
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. The latter feature shows that weak binding sites, similar to those induced in azurin by the destabilizing salt KSCN (12), are common with proteins under these conditions
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. To aid comparing the ANS binding propensity among the various proteins Table 2 lists the minimum number of binding sites (n) as well as the ratio n/Mw, normalization by the molecular weight taking into account that larger macromolecules, such as oligomers, have a greater probability to bind ANS simply for their larger mass
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. Effects of sucrose on the internal dynamics of azurin
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. 1 B and by their independence of the scan rate), the heating and cooling curves of the apoA-2:DMPC complexes show hysteresis that was also observed in DMPC complexes with apoC-1 (Gursky et al
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. Evidence for a unique folding intermediate in the 4WJ ribozymeOur results indicate that the SL 4WJ ribozyme can fold into a compact conformation in the presence of high Mg^sup 2+^ concentrations, with arms A and B juxtaposed in a similar fashion to the docked native 4WJ ribozyme
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