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. " It is quite possible that even he may not know for sure whether he has molded his views to suit anti-Israeli donors; whether his center has attracted the money of such donors because they like his views; or whether, while fighting the guinea worm, he simply made the unrelated mistake of writing an appallingly biased and bad book
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. Intrinsic fluorescence spectroscopy The samples for the fluorescence measurements were prepared by dissolved ACYI in 30 mM Tris-HCl (pH 7
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. Importance of the carboxyl-lemiinal domain of enzyme I of the Eschericliia coli phosphocnolpyruvate:sugar phosphotransferase system for phosphoryl donor specificity
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. As with many concepts in biology, the notion that death, removal, and rebirth are integral to the adult organism is far from new
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. A possible explanation for the above correlation is that the drastic drop in water activity on ice formation causes the cavities to dehydrate and collapse with consequent rearrangement of the internal peptide structure
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. Once protein unfolding reached completion in the first jump, the sample was cooled and incubated at 25°C for 1000 s, which was sufficient for completion of the helical refolding (as indicated by flattening of the Θ^sub 222^(t)) but not for the disk reconstitution (as indicated by DSC, EM, and gel electrophoresis)
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. ofYear Cruise DatesstationsIchthyoplankton surveys1978-79 SM-IX 26 Dec-07 Jan 281982EH-05/8219 Nov-03 Dec 651983EH-01/8314 Jan-26 Jan 431985OB-02/8525 Mar-04 Apr 301986OB-01/8620 Jan-03 Feb 40OB-07/8609 Dec-22 Dec 431991OB-07/9101 Nov-11 Nov 351995OB-14/9505 Dec-18 Dec 751996EH-17/9612 Dec-21 Dec 181998OB-10/9807 Dec-20 Dec 871999OB-09/9911 Dec-17 Dec 152000OB-14/0009 Dec-21 Dec 272001EH-01/0106 Jan-29 Jan 28OB-02/0112 Feb-25 Feb 40OB-13/0110 Nov 13 Nov 18Posttransition juvenile trawls1992EH-02/9202 Mar-21 Mar 451998EH-04/9801 Apr-10 Apr 411999EH-04/9920 May-31 May 562000OB-05/0001 Jun-20 Jun 1122001OB-02/0112 Feb-25 Feb 23Lat
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. t] x t [[gamma]
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- Without the infusion of cash, Myers said, his small congregation probably would have closed its doors
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. In high concentrations of denaturants, proteins behave like polymers in a good solvent and ensembles for denatured proteins can be modeled by ignoring all interactions except excluded volume (EV) effects
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. Rather, structures need to be associated to other bodies of data, putting them into specific biological context
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. Our models were in all cases (500 runs) unsuccessful, since the distance between both sites was very large to allow for the transference of the phosphate group (>10 [Angstrom])
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. This feature can either by related to arising tyrosine emission (often observed around 305 nm for unfolded states when energy transfer to tryptophans disappears during a structural expansion of the protein; see, for example, Schmid (20)) or to elastic scattering caused by aggregation
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. This method uses the extended Lipari-Szabo approach (23) and fully anisotropic diffusion to derive motional parameters from relaxation data measured at two magnetic fields
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.We thank Janny Hof for critical reading of the manuscript
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. However, more important conclusions arise when our results are compared with previous reports on DNA binding with monocations
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.5), which is necessary for the glutaraldehyde reaction
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., 1998) and to elucidate the global geometry of the hairpin ribozyme in docked and extended states (Murchie et al
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. Since we use relative peak positions and distance (inter-arm proximity) changes in our discussion here (and not absolute distances), variations of [gamma] from 1 do not affect the discussion or conclusions of this study
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