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Structural rearrangements then take place at the dimer interface (and presumably also in other domains of each subunit), leading to a final, compact dimer structure
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Surprisingly, little is known about these alterations, but it has become clear that the externalization of phosphatidylserine (PS) is an essential element
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Proteins in frozen solutions: evidence of ice-induced partial unfolding
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Secreted DmAChE was purified to homogeneity using the following steps: 70% ammonium sulfate precipitation, dialysis, affinity chromatographies on a procainamide-Sepharose column and on nitrilotriacetic acid-nickel agarose (39)
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With the method of Loria et al
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Although this value of specific volume is required to approximate the viscous behavior at intermediate protein concentrations, the specific volume used for approximating the concentration-dependent osmotic pressure (1
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0 no salt), and soluble misfolded oligomeric state (pH 2
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Initiation of oligomerization was performed through addition of 100 mM NaCl from a 4 M stock solution to 0
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Suzanne ScarlataDepartment of Physiology & Biophysics, State University of New York at Stony Brook, New York 11794-8661Submitted November 4, 2004, and accepted for publication January 18, 2005
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Although it is known that parts of the insulin chains, such as the C-terminal of the B-chain, are not expected to participate in the tight intra- and interfibrillar packing (therefore giving rise to loose and soft elements protruding alongside the protofilament-Jimenez et al
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For proteins containing N residues, there are of order N^sup 2^ nonnative interactions that may be encountered during a diffusive search, but only of order N interactions that define the native state
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Such a machine can allow the same feed rates and speeds to be used as in wet machining processes
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This method was successfully tested for biomolecular simulations of peptide folding (Schäfer et al
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The crossing-over of the complex and free buildup curves at ~6 ns (Fig
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Irregularly shaped particles that could not be divided into spherical particles were produced for cases D and E (similar cooling rates)
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