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Computational studies on the glycan-protein interactions at the noncanonical interface of the protein show that quite a number of hydrogen bond and hydrophobic interactions stabilize the glycoprotein tetramer
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The addition of PEP slightly destabilized EF^sup sc^, and the presence of Mg^sup 2+^ stabilized it
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Other probable environmental factors include the following: (1) low levels of SP-A, SP-D, lipoxins, and annexin I; (2) high levels of TNF-[alpha] (K
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htm, developed by Syracuse Research, Syracuse, NY) for the method of Meylan and Howard (28)
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5 ns with squared order parameter (S^sup 2^) of ~0
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Spectra from the relaxation-compensated CPMG experiments were integrated and analyzed in the same manner as the R^sub 1^ and R^sub 2^ experiments
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One round of water rinsing was performed applying 5 µl water
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The Gβγ-subunits used in this study are geranylgeranylated, whereas Gα^sub s^-subunits are dually palmitoyated (Kleuss and Krause, 2003: Wedegaertner et al
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This suggests that two slightly different conformational spaces are sampled in the free and complex forms of the molecule, representing a situation of case A (Fig
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The donor-acceptor solutions used were 100 pM in donor concentration, ensuring that virtually all of the detected signals were due to single molecules
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This duplex has a 14-bp separation between donor and acceptor, and spFRET experiments show only a small change in E upon going from 0
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