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. Details with regard to the intrinsic fluorescence experiments were the same as those described before (30)
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. RESULTS In this work, we focus on two-state proteins because the hypothesis is that only two well-defined macrostates-native and highly denatured states-are accessible to these systems (67-70)
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. This study was designed to evaluate the potential of localized MR spectroscopy to investigate subcellular phenomena
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. Our main goal with this study was to employ a variety of fluorescence probes (ANS, Bis-ANS, DCVJ, and ThT) and techniques (steady state and time resolved) to recognize and characterize different states of a protein in the process of misfolding and self-assembly
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. We remark that in both these cases, the stabilized hypergutter provides an arena in which "diffusion-collision" calculations can operate within a molten globule, so constituting a significant generalization of that model to nonspatial degrees of freedom (Zhou and Karplus, 1999)
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. When key stabilizing interactions succeed in reducing the dimensionality of the search space more drastically, much higher values can result (from differentiation of the higher-dimensional analogs of Eq
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. The first is that special tuning of nonnative interactions may contribute significantly to rapid folding; they stabilize the hypergutter-potentials that keep diffusive search dimensions under control
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. 041Effect of Type 2 RPCumulative effect over all months $4,637,901<
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. Table 2Means and Standard Deviations of Intrinsic and ExtrinsicMotivation Scales forParticipants Who Reported Having Gambled In the PreviousSix Months Men WomenTotal (N = 62) (N = 61)(N = 123) MSD MSD MSDIntrinsicTo Know 2
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. Since the MG conformation is more flexible than a fully folded polypeptide, this could lead to better packing at the dimer interface, leading to more stable dimers
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. However, those authors propose that this phenomenon is unlikely to have physiological relevance, since the inactivation of GR is very slow at cellular NADPH concentrations
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. For the residues with significant exchange broadening, specific k^sup -1^^sub ex^ values were derived (Table 1)
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. The date acquisition card settings were chosen to give a time resolution 10-100 ps depending on the decay time of the fluorescent probe in question
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. To further evaluate the contribution of solvent-induced shifts, we examined a model duplex DNA molecule whose structure is not expected to change significantly with Mg^sup 2+^ concentration changes in the 0
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