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The fluorescence emission of GR undergoes a marked red-shift between 1 and 3 M GdnHCl (with a transition midpoint at ~2
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These two sets of researchers use either coil library statistics (125) or conformations of residues in loops (114) to model local, sequence-context-dependent conformational preferences
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Hvdrodynamic radii of native and denatured proteins measured by pulse field gradient NMR techniques
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Here, the much greater intensity enhancement observed for the mutants, compared to the WT, stands to indicate that the number of ANS binding sites and, consequently, the magnitude of the structural alteration are sensibly larger with these less stable azurins
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In conclusion, from examining azurin mutants of lower thermodynamic stability and greater internal flexibility than the WT, it was found that the perturbation of the native fold in the frozen state, evidenced by the capacity to bind ANS, is enhanced by the plasticity of the globular structure and correlates with the lability to freeze-thawing
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7) are all located in α-helices, whereas the residues with f [asymptotically =] 0
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In this study, we determined the volume change for Gβγ-PLCβ interactions in solution and bound to membranes
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, 1995), and our previous work has shown that a peptide corresponding to one of the Gβ blades, Gβ86-105, binds to and activates PLCβ, whereas peptides that correspond to other blades may bind but not activate PLCβ (Buck et al
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The thermodynamics of the self-association of the reduced and carboxymethylated form of apoA-II from the human high density lipoprotein complex
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Based on the temperature determined by a probe, an automatic compensation was made to the position of the feature machined
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In summary, our comparative study of different ribozyme constructs shows that in contrast with the 2WJ and the 3WJ, the natural 4WJ architecture can efficiently guide conformational search events during ribozyme folding, thereby dramatically influencing subsequent tertiary structure formation and stability
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Most of the savings from the introduction of RP were therefore attributable to substitutions of low cost unrestricted for higher cost restricted NSAIDs
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The facility could hold about seven football fields, and is home base for 1,200 employees, including 140 full-time pharmacists, who work three shifts a day, six days a week
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Because such models do not consider players' reasoning or cognitions (e
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