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. The decrease in the content of Class I Trp fluorophores suggested that the intermediate had a loose tertiary structure, which allowed the penetration of water molecules into the hydrophohic interior
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. The influence of inhibitor-binding on global enzyme molecular dynamicsAlthough several studies using different techniques have shown a modification of global protein molecular dynamics on an Ångström-nanosecond scale upon inhibitor-binding, we were not able to detect any significant differences between the native and the inhibited HuBChE, at comparable degrees of hydration, up to ~334 K (61°C)
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. This work was supported through the core strategic grant of the United Kingdom Biotechnology and Biological Sciences Research Council
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. Molecular Biology of the Cell
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. This can be concluded from the subsequent change after appending the trajectories of the complex and of the free form to each other, which is consistent with the previously defined case A (Fig
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