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Phase diagram analysis of IR and fluorescence data The phase diagram analysis, which is a sensitive tool to detect folding intermediates, was carried out as described previously (35)
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Average shapes are consistent with prolate ellipsoids
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The main point of the foregoing discussion is that inasmuch as there is congruence between the EV-limit ensemble and highly denatured states, chain fluctuations create ample room to accommodate favorable interactions with the surrounding solvent
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Manufacturers are responding to these needs with great-tasting products with major reductions in "bad" features like trans fats and calories, and significant additions of "good" features like antioxidants and whole grains
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The best tit was obtained by using the two-state model
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whether the T^sub m^S are the same as at pH 7
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In principle mainly two properties of the fluorescence emission exhibit the most pronounced signal changes upon conformational changes of the protein
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At lower pHs an increase in ionic strength screens the electrostatic repulsion of positive charges on the monomer and stabilizes the dimer (25,26)
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Purification was performed as described previously (Lashuel et al
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In this case, the "funneled landscape" appears in the spatial coordinates themselves
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Lepore, a 21-year Charles Schwab veteran who helped design Schwab s technology platform and played a key role in growing its online brokerage business, succeeds Kal Raman, who stepped down as chief executive officer in June after growing revenues to $246 million in 2003 from $145 million in 2001
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Journal of College Student Development, 37(1), 20-28
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