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. The decreased fitness often leads to early death or a low rate of reproduction, thus eliminating the harmful mutation
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. Parameter A, which reflects the spectral shape of the intrinsic Trp fluorescence (31), was obtained by dividing the fluorescence intensity at 320 nm by the intensity at 365 nm
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. However, it may also happen that at this denaturant concentration, some amount of protein has reached the completely denatured slate and the loss in the ellipticity value is due to the contribution from that fraction of the denatured protein
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. Since the tryptophan is at the C-terminus of EI^sup SC^
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. This ~90° rotation of the diffusion tensor together with the relatively small structural changes within each CaM domain puts direct restraints on the overall structure of the CaM:SEF2-1mp complex, which allows us to choose one of the two possible schematic structure models proposed in our earlier study (11) (see Figs
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. The origin of this difference arises from the estimation of φ from mass fraction
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. As for the biological aspects of this work, the noted high potential of Na+ cation in DNA compaction supports the notion that Na+ ion (contrary to K+) plays an important role in maintaining the compact higher order structure of DNA in vivo
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