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. No changes in anisotropy were observed up to 2 M GdnHCl
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. Protein measurement with the folin phenol reagent
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. To better visualize the sequential events occurring during ACYI thermal unfolding, 2D IR synchronous and asynchronous plots were constructed
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. It has been proposed that the catalytic domain contains a β-sheet sandwiched between α-helices and a second β-sheet located on the surface, whereas the dimerization domain consists of a β-sheet flanked on one side by two α-helices (17)
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. These two sets of researchers use either coil library statistics (125) or conformations of residues in loops (114) to model local, sequence-context-dependent conformational preferences
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. Biochem Biophys Res Commun 2004: 318: 32-37(52) Huynh ML, Malcolm KC, Kotaru C, et al
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. However, more meaningful data might be obtained by employing encapsulation techniques, which in principle can ensure studies without interfering interactions of tryptophan residues with the encapsulating material
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. 3, A and B, are characteristic of the latter protein class
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. californica neuron (15), which brought about a negligible lipid signal and large amounts of other metabolites, including the neurotransmitter choline
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. Below we consider equations assuming fast exchange
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. The theoretical equation of state does not describe the experimental results as well as at pHs 8
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. Further maturation of the aggregates showed shrinkage of the spheres as the fibrils grew in length, suggesting a conformational change of the spheres into more rigid structures
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. It is notable that the saturated palmitoyl chains would be expected to become more stabilized as the membranes enter the gel phase, but the opposite is observed
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. The features can be classified as: parallel tubular fibers, twisted ribbonlike structures, rod bundles, and ropelike textures
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. One can, for example, resolve the entropic contributions of hydrophilic and hydrophobic residues to protein-protein binding, which is experimentally impossible
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. J Orthop Res 1990:8:266-75
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. Most of these studies have been performed in medical, dental and nursing students (Beck, & Srivastava, 1991 ; Coburn, & Jovaisas, 1975; Davis, Tedesco, & Meier, 1989; Hamill, 1995; Helmers, Danoff, Steinert, Leyton, & Young, 1997; Vitaliano, Maiuuro, Mitchell, & Russo, 1989)
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. The changes of thermodynamic parameters (enthalpy change ((Delta)H), entropy change ((Delta)S) and heat capacity change ((delta)C^sub p^)) corresponding to the transitions from N to MG (molten globule state) or MG to U (unfolded state), were summarized in the previous report (Nishii et al
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. Based on mass spectromctric results, a structural model of ACYI was proposed, and it was suggested that each ACYI subunit might be composed of two domains with α/β folds: the catalytic domain and the dimerizalion domain (17)
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., limited to the active site), concern low-frequency collective modes below the sensitivity of the technique used and/or present only on a longer timescale (> 1 ns), and not directly related to fast thermal fluctuations
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. The addition of a small amount of Bi (1 wt
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. Furthermore, information on the effect on folding rate of replacing specific residues via mutation or "Φ-value" analysis (Fersht, 2000) needs to be taken together with correlations of contact order
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.Several general predictions follow
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. We conclude that the critical melt flow rate sufficient for continuous film formation is somewhere between 0
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. Docking of the two loops is also observed in simpler ribozyme constructs comprised of two- or three-way helical junctions (2WJ and 3WJ, respectively), although much higher Mg^sup 2+^ concentrations are required (N
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