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Biologists today endorse two principal but competing hypotheses to account for the evolution of degenerated eyes
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The activity decreased rapidly at higher temperatures (>65°C) due to inactivation of the enzyme
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Based on these findings, it is tempting to suggest that the changes monitored by far-UV CD were due to the proximity of residues in the tertiary structure of the whole intact EIC^sup sc^ structure, but not of the isolated domains (and then, in those modeled in this work)
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Lancet 2005; 365:1552-1560(63) van der Vaart H, Koeter GH, Postma DS, et al
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However, the herein-observed morphological variation after increasing temperature bears a more profound meaning in light of the ongoing debate as to the identity of the protein's aggregation-prone conformation (completely unfolded, or partly unfolded protein) and our own study, showing that under similar conditions, bovine insulin is substantially more unfolded at 70 than at 60°C (Dzwolak et al, 2003)
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BD=body depth;BL=body length; ED=eye diameter; HD=head depth;HL=head length; PAL=preanal length; PDL=predorsallength
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. These difference maps are shown in Fig
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. no histidine residue was near to any of the tyrosine residues or Trp^sup 461^
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