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. Moreover, the strategies used in this research might also facilitate the characterization of the potential pretransitional changes in the thermal transitions of other thermostable or thermophilic proteins
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. Our results show that the secondary structure of EI^sup sc^ was not substantially altered, as suggested by FTIR experiments, but quenching experiments and proteolysis patterns suggest that there were small conformational changes upon binding of PEP; in addition, the thermal stability of EI^sup sc^ was slightly modified when any effector was added
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. Rather solvent accessible tryptophan residues in the folded state (Fig
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. This feature can either by related to arising tyrosine emission (often observed around 305 nm for unfolded states when energy transfer to tryptophans disappears during a structural expansion of the protein; see, for example, Schmid (20)) or to elastic scattering caused by aggregation
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