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4 µM) were incubated for 90 min in the absence or in the presence of different GdnHCl concentrations and applied onto a Superdex 200 HR column (Pharmacia, Uppsala, Sweden), previously equilibrated with 20 mM Tris-HCl, at pH 7
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Nuclear magnetic relaxation studies of the role of the metal ion in Mn^sup 2+^-substituted aminoacylase I
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Fourteen-member macrolides promote the phosphatidylserine receptor-dependent phagocytosis of apoptotic neutrophils by alveolar macrophages
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Due to a rather slow structural unfolding but a fast irreversible step we observe an unfolding process which is under kinetic control
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The lowest protein concentration of 7 µg/ml reveals a large red shift with λ^sub max^ of ~345 nm, whereas protein concentrations of 50 µg/ml and 1 mg/ml result in λ^sub max^-values ~340 nm for the unfolded states
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6 as a horizontal line indicated with the letter "U" in different colors corresponding to the probe
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The most rapid components had lifetimes Time-resolved fluorescence anisotropy was employed to probe the dynamics of different conformational states of TTR
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Surprisingly, the rank order for the disk stability indicated by our DSC, CD, and light-scattering data (Figs
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This is followed by the binding of the chemokine co-receptor to gp120 (Berger et al
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To better understand the basis for the observed broadening and tailing, we carried out numerical simulations using a simple model of the single-molecule FRET data
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t] x t [[gamma]
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