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The precipitate was resuspended m 6 M guanidinium hydrochloride (in 10 mM Tris buffer (pH 7
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Coming to the nature of the interactions, we see that both hydrogen bonding and hydrophobic interactions prevail in the system, the frequency of the latter occurring more often
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Although for BSA a high binding capacity may be expected from its intrinsic tendency to bind nonpolar and aromatic moieties also in the liquid state, the above ANS binding data do suggest that beyond specific trends of individual polypeptides, multi-subunit proteins are more susceptible than monomers to destabilization and partial unfolding in the frozen state
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Wetting and Reaction of Sn-2
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The CaM:SEF2-1mp complex is found to be highly flexible with internal motions on the picosecond, nanosecond, and microsecond timescales
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The predicted dependence of η^sub r^ on mass fraction for particles with a v of 3
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Interestingly, we found that ANS fluorescence intensity increases as the A-state molten globule of TTR aggregates
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This work was supported by grants from The Nanotech Programme of the Swedish Defence Research Agency (M
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Other patterns of native and nonnative cooperativity could favor other orderings
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Journal of Zoology 148:201-217
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By contrast GAG levels were reduced by 25% over the same period for pellets maintained in DMEM/10%FCS
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"McClellan said the agency would release a revised definition of AMP later this year for public comment, along with an initial round of AMP data, based on the new definition
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