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. Thermal denaturations following the ANS fluorescence did not show a native baseline, and a sigmoidal-like decreasing of the fluorescence was observed as the temperature was raised (data not shown)
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. For magic angle spinning (MAS) experiments, an indirect detection pulsed field gradient nano H{X} probe (4 mm) was used in a Varian (Palo Alto, CA) UNITY INOVA 500 NMR Spectrometer
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. RESULTS AND DISCUSSIONCharacterization of soluble prefibrillar aggregates and protofibrilsGeneration of prefibrillar oligomers of TTR from the A-stateProlonged dialysis of TTR (96 h, 4°C) versus 10 m M HCl in dH^sub 2^O in the absence of salt (pH 2
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. For membrane-bound proteins, we expect a similar dissociation mechanism, assuming that pressure does not alter the properties of the membrane surface or promote dissociation of the membrane-bound proteins
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. MATERIALS AND METHODSLipoprotein preparation and characterizationSingle-donor HDLs were isolated from EDTA-treated human plasma by density gradient centrifugation in the range 1
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. This suggests that the protein refolding detected by CD between 32 and 24°C upon relatively rapid cooling (Fig
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. The dialyzed solution was centrifuged at 8000 rpm for 15 min and the clear supernatant was loaded on a lactosylamine Biogel P-150 column preequilibrated wilh buffer (21)
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. However, the absence of native baseline at low temperatures did not yield reliable results when fitting the data to Eq
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. The Cu^sub 6^Sn^sub 5^ compounds also appear in the solders other than the solder/Cu interfaces when the concentration of Cu into the molten solder reaches the threshold limit of Cu^sub 6^Sn^sub 5^ formation
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. This interaction perturbs the contacts that Gβγ makes with PLCβ, which causes enzyme activation
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