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The heat flow versus temperature profile of ACYI contained an asymmetric positive peak arising from an exothermic transition (Fig
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This modeled structure has allowed us to explain the experimental conformational and thennodynamic features of the protein
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Several NMR variants additionally provide spatial information (2,3): Spatial maps of water protons, relaxation properties, diffusion or flow inside an animal or human body can be obtained with magnetic resonance imaging (MRI), and in vivo localized magnetic resonance spectroscopy (MRS) delivers chemical spectra at predefined regions of such samples
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Surprisingly, the rank order for the disk stability indicated by our DSC, CD, and light-scattering data (Figs
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Characterization of the oligomeric states of insulin in self-assembly and amyloid fibril formation by mass spectrometry
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The low number of positive stations in spite of the intense sampling conducted within the area of distribution of P
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The low molecular weight IPP polymers produced smaller particles than the high molecular weight polymers
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Risk spreading as an adaptive strategy in iteroparous life histories
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More recently, single-molecule FRET detection (Ha et al
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. Interdomain interaction and substrate coupling effects of dimerizalion and conformational stability of Enzyme I of the Esclierichia coli phosphoenolpyruvate:sugar phosphotransferase system
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.MATERIALS AND METHODSEnzyme purification and characterizationHuBChE was purified to homogeneity from outdated human plasma according to an improved protocol based on that of Grunwald et al
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. If this idea is correct, then the volume associated with Gβγ activation of PLCβ corresponds to the difference in volume changes for PLCβ-Gβγ alone and in the presence of Gα^sub s^(GDP), ranging from ΔV ~3 to 15 ml/mol (Table 1)
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. The two fibers marked with the green and blue arrows are of the comparable height of 6
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. In the triple-helix example that we looked at in detail, the nonnative hydrophobic strips that accelerate the final two-dimensional search are "activated" only when the helices themselves form, at least partially (by the formation of some native interactions) and when their axial rotational degrees of freedom align
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. semifasciata suggests a reduced spawning site
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