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Protein expression and purification The EI^sup sc^ clone comprises residues 1-576, with an extra methionine and a His^sub 6^-tag at the N-terminus (15)
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This shape did not change as the pH was modified, except in the region between 3
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The results of the trypsin digestion and quenching experiments qualitatively agree, indicating that EP suffers conlbrmational changes upon binding to PEP
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In this case, however, stabilization of the native state is most probably due to a decrease in free energy of the native state; phosphate, like sulfate, is a salting-out ion that stabilizes folded protein structures and favors aggregation and precipitation
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Thus, the data are consistent with a motional model in which the two N-terminal and two C-terminal CaM domains in the dimeric CaM:SEF2-lmp complex undergo a small-scale wobbling motion with a half-angle of ~20° as estimated from S^sup 2^^sub s^ = 0
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Hence, there are four hinge regions in the CaM dimer
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Consequently, the on-rate of the equilibrium between free and CaM-bound peptide will be faster than diffusion controlled, which explains the fast exchange and the high affinity observed in the CaM:SEF2-1mp interaction (11)
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However, due to the close proximity of all involved components, a new CaM:SEF2-1mp interaction is rapidly reformed, which in turn forces the CaM dimer to close again
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Therefore, the difference between DCVJ and ThT is that ThT does not fluoresce in the presence of protofibrillar amyloid of TTR at pH 3
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Elsewhere it was shown that insulin fibrils' morphologies and the corresponding infrared spectra vary depending on conditions of their growth (Nielsen et al
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In the combined trajectory analysis (Fig
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[FIGURE 3, 4 & 5 OMITTED]FecundityAbsolute fecundity for prefertilization female black rockfish ranged from 482,528 oocytcs for a 5-year-old female to 998,050 oocytes for a 19-ycar-old female
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1% (w/v) CaCl^sub 2^ in distilled H2O at 37°C for ten minutes
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There is some precedent for this in the Australian state of Victoria, where officials watch for adverse effects while drugs are sold only in pharmacies and then consider whether to shift a drug to full nonprescription status
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