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According to this view, the tight and specific association of subunits in the native GR dimer may be facilitated by contacts between MG monomers
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Moreover, resolution-enhancing techniques, such as second derivative and 2D IR, have been shown to be sensitive tools to monitor the sequential events in protein conformational changes by FTIR (27,28,30,38-42)
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Since the frequency of the solvent-exposed secondary structures is smaller than the buried ones (44,45), the above sequential events could be interpreted as follows: the unfolding of the solvent-exposed β-sheet structures is the earliest event, followed by the formation of aggregates, and finally the unfolding of the other native structures
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These hierarchical distance distributions reflect the so-called dilatation symmetry in the EV limit whereby contact density maps for one protein sequence can be rescaled to obtain the contact density map for another sequence
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In C elegans, two signaling pathways govern corpse removal; both possibly converging at a common effector, ced-10 (mammalian homolog Rac-1)
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Respir Med 2005; 99:1175-1182(65) Berry MA, Hargadon B, Shelley M, et al
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Irreversible thermal denaturation of Torpedo californica acetylcholinesterase
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Thus, in ice either the high affinity ANS binding sites present in solution are no longer available to the aromatic probe or the fluorescence yield of ANS bound to these sites is drastically reduced
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The relaxation data at 600 MHz were integrated over an elliptically shaped area with diameters 7
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A total of 25 µl 6 × SDS sample buffer (final SDS concentration of 2
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Upon maturation the oligomers clustered into either protofibrils or spherical aggregates, and upon further maturation the spheres shrink in size as fibrils extend in length
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Transthyretin aggregation under partially denaturing conditions is a downhill polymerization
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British Museum (Natural History), London, UK
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A 100 µl sample of Hoechst (1 µg/ml) was added to each of the wells
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