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The supernatant was again subjected to 65% ammonium sulfate fractionation
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INTRODUCTION The development of an accurate theoretical framework for describing denatured-state ensembles of proteins has been a topic of long-standing interest (1-12)
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(104) ignored denatured-state topologies when quantifying the correlation between native-state topology and folding rates
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with T^sub m^ close to 330 K (Fig
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Therefore, the respective unfolded states can have rather different structures (21)
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The fluorescence emission, collected at 90° from the excitation, is dispersed by a 0
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The exchange broadening must then be due to conformational exchange between different conformations of bound CaM
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Redistribution and loss of side chain entropy upon formation of a calmodulin-peptide complex
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9 nm in diameter) partially unfolded spherical oligomers are apparently capable of merging into the amyloid precursor
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Universidad National del Sur, Bahia Blanca, Buenos Aires, Argentina
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The samples were treated in papain digest buffer, as described above
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