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. Abbreviations used: CD, circular dichroism; DTNB, 5,5'-dithiobis-(2-nitrobenzoic) acid; EDTA, ethylenediaminetetracetic acid; GdnHCl, guanidine hydrochloride; GR, yeast glutathione reductase; GSH, reduced glutathione; GSSG, oxidized glutathione; HEPES, n-(2-hydroxyethyl) piperazine-n'-(2-ethanesulfonic acid); HPLC, high performance liquid chromatography; MG, molten globule
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. In addition, a 10-Å distance-based cutoff was applied between all nonbonded atoms
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. In the EV limit, there exists a so-called "correlation hole" (39)
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. The PK^sub a^, leading to the acidic species could be determined from the ANSbinding and intrinsic fluorescence measurements, suggesting that one aspartic and/or glutamic acid must be responsible for this transition (43)
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. Folding and association of oligomeric and multimeric proteins
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. (13,16,38) The suppressive effect of efferocytosis on the adaptive immune system appears to be largely PS-dependent, to include both TGF-[beta]1-dependent and TGF-[beta] 1-independent mechanisms, and to result in decreased antigen-specific CD4 T cells, antigen-specific IgG, dendritic cell maturation, and IL-12 production
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. Therefore, the difference between DCVJ and ThT is that ThT does not fluoresce in the presence of protofibrillar amyloid of TTR at pH 3
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. Steady-state FRET methods were first used to monitor the docking of loops A and B during magnesium-induced folding (N
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- Our target was 10 percent
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.GMAC Commercial Mortgage CorporationGMAC Commercial Mortgage Corporation arranged a $4
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. This limit is of interest in light of data that suggest the presence of residual structure even under strongly denaturing conditions
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. For each of the sequences shown in Table 1
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. This key regulator of cellular integrity controls the transcriptional expression of downstream cell cycle regulators, as well as the interactions between the actin cytoskeleton and cadherin within the adherens junctions
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. Below we consider equations assuming fast exchange
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. In fact, the pattern of (τ^sup o^^sub m^)^sub app^ back calculated from the CaM:SMLCK complex when the diffusion tensor would be oriented along an axis perpendicular to the real diffusion tensor closely matches the pattern of (τ^sup o^^sub m^)^sub app^ found for the dimeric CaM:SEF2-1mp complex, Fig
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. The presence of exchange between multiple-target sites and flexibility in the bound state leads to an important advantage over rigid binding, namely that the affinity (and specificity) is finely tuned
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. This was possible due to the exploration of a novel pathway of TTR aggregation from the molten globule A-state, affording soluble assemblies of misfolded protein molecules
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.Size and morphology of special aggregates and protofibrilsAliquots from a TTR aggregation reaction solution taken after 30 min, 2 h, 24 h, and 430 h were investigated using transmission electron microscopy (Fig
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. Three probes-ANS, DCVJ, and ThT-show increased fluorescence throughout the aggregation reaction, whereas Bis-ANS shows fluorescence decay (Fig
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. An alternative, machining with "minimum quantity lubricant," or MQL, is gaining acceptance as a cost-saving and environmentally friendly option in place of some wet machining processes
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.FORMATION OF THE EVA TONERSThe process conditions were similar to those for IPP: 10 wt% polymer concentration, 2-hour dissolving time, Isopar G solvent, and cooling in a water/ice bath of 4°C
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. Vets there use several NSAIDs that the new study links to bird deaths
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.3 percent for Asian/Pacific Islander Americans, while Latino/Hispanic graduates comprised only 2
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.--Pharmaceutical Science* MICHIGANUniversity of Michigan College of Pharmacy Ph
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