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. Similarly to that observed for EIC^sup ec^, the electrostatic, as well as the van der Waals interactions, were decreased in EIC^sup sc^, losing several salt bridges and hydrogen bonds when compared with the structure of EIC^sup tt^
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. Similar results have been observed in 1), the enzyme malate synthase G (54), a close structural analog of pyruvate dikinase and EIC (11), where binding to pyruvate does not cause large structural changes (55); and 2), the pyruvate dikinase from maize, where only small changes are observed in selected regions upon binding of PEP (56)
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. Besides the shift of transition temperatures caused by different heating rates, pronounced differences in the λ^sub max^-values for the unfolded states are visible in Fig
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