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. 4 strongly supported the conclusion that pretransitional conformation changes occurred at temperatures where the enzyme retained high activity (see Fig
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. Fifty scans per temperature were acquired and averaged
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. The three-dimensional structure of BChE is very similar (21)
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. Modifications induced upon oligomerization in high-frequency, small amplitude/localized modes, which do lie within the sensitivity of our experimental window, might be rather small
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. ANS bound to native TTR showed a major component with a lifetime of 16
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. Regulation of the rate and extent of phospholipase C-β^sub 2^ effector activation of the βγ subunits of heterotrimeric G-proteins
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. The formation of LpA-1/A-2 has been proposed to involve LCAT-induced fusion of the precursor particles containing apoA-1 and apoA-2 (Clay et al
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. Mature human plasma HDL and LDL are also stabilized by kinetic barriers, suggesting that such barriers provide a universal natural strategy for lipoprotein stabilization (Mehta et al
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. Far-U V CD spectra (185-250 nm) were recorded from solutions of 20 µg/ml protein concentration placed in 5-mm-pathlength cells, which are standard condition in our CD experiments
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. Rationalization of this observation has given rise to a third view of the critical pathway of protein folding, the "topomer search" model (Plaxco and Gross, 2001)
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. Overall, men did report significantly higher levels of intrinsic involvement than women
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