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NH^sub 2^-terminal sequence truncation decreases the stability of bovine rhodanese, minimally perturbs its crystal structure, and enhances interaction with GroEL under native conditions
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(12) reported localized B-factor reductions within the substrate binding site in serine protease A upon binding of an inhibitor
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The PLC-γ1 C-terminal SH2 domain (19) and Csk SH3 domain (47) bind specifically to their flexible (peptide) target to form a specific complex, again with essentially one overall conformation
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Mapping the folding intermediate of human carbonic anhydrase II
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Most of the residues of CD4 and gp120 that are involved in the intermolecular hydrogen-bond network (solid bars in Fig
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--Cell Biology and Biotechnology Health Psychology Pharmacy Administration Pharmaceutical Business (MBA) Bioinformatics Biomedical Writing MS
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.5 M denaturant concentration also exibits a loss in the value of the mean residue ellipticity, suggesting that there is a loss in the secondary structure of the protein (Fig
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.More recently, we have found that CFTR deficiency directly impairs efferocytosis by epithelial cells (unpublished data), an effect that may be related to its membership in the ABC transporter superfamily (such as ced-7, ABC-A1, and ABC-A7)
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. One can, for example, resolve the entropic contributions of hydrophilic and hydrophobic residues to protein-protein binding, which is experimentally impossible
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. In addition, our previous MD analysis of the intermolecular hydrogen bonding shows that all these residues, except CD4-K22, form only marginally stable intermolecular hydrogen bonds
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.AcknowledgmentsThis study resulted from cooperative efforts of the USGS Biological Survey Unit and the Department of Zoology, National Museum of Natural History
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