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. Circular dichroismFar-UV CD spectra of GR were measured on a JASCO (Tokyo, Japan) J-715 spectropolarimeter (kindly made available by Dr
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. CD measurements suggested that unfolding of GR was complete at 7 M GdnHCl, a result that was consistent with the iodide quenching data
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. For reasons to be discussed below, this assertion is in fact inaccurate
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.ChEs provide a particularly suitable system for studying relationships between activity, ligand binding, unfolding, and thermal molecular fluctuations due to the following properties:1
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. Nature has thus found a way for CaM to specifically recognize a relatively ill-fitting target in its regulation of bHLH transcription factors
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. A comparative x-ray diffraction and circular dichroism study of DNA compact particles formed in water-salt solutions, containing poly (ethylene glycol)
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. Aliquots of the aggregation reaction were withdrawn and mixed with an assay buffer (10 mM HCl, containing a final concentration of ANS, Bis-ANS, or DCVJ at 2
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. There are four families of Gα-subunits, and phospholipase C-β(PLCβ) is the main effector of the Gα^sub q^ family (Rebecchi and Pentyala, 2000; Rhee, 2001)
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. In summary, chemical denaturation of discoidal lipoproteins is a complex multiphase transition whose kinetics is distinctly different from that of the thermal denaturation; similar to thermal denaturation, chemical denaturation involves protein unfolding/dissociation (Reijngoud and Phillips, 1982) and lipoprotein fusion (Fig
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. Independently from producing evidence for the step-resolved character of the process, intermediate and morphologically heterogeneous forms were trapped and characterized, which yields direct evidence for the multipathway character of the amyloidogenesis of insulin
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.For links to both of these articles, visit www
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. In the combined trajectory analysis (Fig
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. Pyruvate (1 mM), dexamethasone (10^sup -7^ M) and 10 ng/ml TGF-[beta]1 (recombinant human, R&D Systems, Abingdon, UK) were also added
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.A more infamous example is the drug thalidomide which was widely and aggressively marketed in the fifties and sixties as "a harmless tranquillizer particularly suitable for the pregnant woman" since it has been shown to be not teratogenic "after repeated and rigorous animal tests (p
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."A very busy retail pharmacy might fill as many as 1,000 prescriptions a week," says Cesanek, Medco's pharmacy practice director, who worked in a small-town retail pharmacy before joining Medco
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.56) and within each professional year (rfirst-year = -0
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