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. The decrease in the content of Class I Trp fluorophores suggested that the intermediate had a loose tertiary structure, which allowed the penetration of water molecules into the hydrophohic interior
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. The implication is that [radical][left angle bracket]R^sup 2^^sub ij^[right angle bracket]~|i - j|^sup v^, where v [asymptotically =] 0
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. The hairpin ribozyme is a good model to explore this question because of the relatively simple secondary structure and the strong dependence of tertiary folding on the helical junction geometry
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. The FRET efficiency histogram of the GnI variant also exhibits two distinct subpopulations, with mean E values that are similar to the wild-type, but the amplitude of the high FRET peak is substantially reduced relative to the low FRET peak (Fig
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- Piper Professor of Biblical Theology at Princeton Theological Seminary
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. The stability of the monomer of rSBA is comparable to many monomeric proteins of similar size
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. The fluorescence emission, collected at 90° from the excitation, is dispersed by a 0
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.Binding affinity of ANS, DCVJ, and ThT to early misfolded TTR oligomersSamples containing 1 µM total concentration of misfolded A-state TTR monomers packed into soluble oligomers harvested 2 h after misfolding initiation were used as bait for probe binding
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. These structures were already ossified in 3
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. The backbone atoms (N, Cα, and C') of the most stable structural elements of both proteins, the first α-helix of gp120 (residues 17-31), and part of the β-sheet of CD4 consisting of residues 26-30 and 82-86 (see Fig
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. An alternative is to divide a trajectory into N blocks of equal size over which sub-averages and standard errors can be calculated; the standard error is given by the standard deviation divided by squared root of the number of sampling points
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. More recently, single-molecule FRET detection (Ha et al
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