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. Unlike the thoroughly studied biochemical properties of ACYI, no high-resolution crystal structure of ACYI is presently available
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. The curves from the diluted solution (data not shown) were similar to those presented in Figs
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. The fact that the HuBChE preparation is a tetramer, whereas the DmAChE preparation is a dimer, does not find expression in a difference in their global MSDs
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. This dependence of the binding of monocations is usually explained from the viewpoint of a decreasing hydrated radius of monocations in aqueous solutions (Ivanov et al
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. Several general predictions result: 1), intermediate subspaces of configurations will always be visited; 2), specific but nonnative interactions may be important in stabilizing these low-dimensional diffusive searches on the folding pathway, as well as native interactions; 3), sequential barriers will commonly be found, even in "two-state" proteins; 4), very early times will show characteristic departures from single-exponential kinetics; and 5), contributions of nonnative interactions to Φ-values and "Chevron plots" are calculable, and may be significant
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. Of course, what we refer to as cavities will actually be filled by solvent and cosolule molecules under denaturing conditions
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. coelicolor, HPr^sup sc^, are different from those of the other members of the HPr family (17-19)
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. We followed a three-part approach
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. the enzyme was more resistant to proteolysis (open and solid circles) (Fig
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. Experiments conducted with copper-free azurin from Pseudomonas aeruginosa and mutants I7S, F110S, and C3A/C26A correlate the magnitude of the ice-induced perturbation, as inferred from the extent of ANS binding, to the plasticity of the globular fold, increasing with less stable globular folds as well as when the flexibility of the macromolecule is enhanced
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.In a first experiment, we compared MSDs of native HuBChE and soman-inhibited HuBChE over a large temperature range (20 to ~349 K), passing the denaturation temperature of native HuBChE
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.This work was supported by National Institutes of Health grant No
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