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. To better understand the step-wise changes of the different Trp fluorophores, the spectrum recorded at each temperature was fitted using the discrete states model of Trp residues in proteins (30,32,34)
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. The importance of native-state topology for folding is underscored by analysis of the average denatured-state topology
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. Statistical coil model of the unfolded state: resolving the reconciliation problem
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. Ice-induced ANS binding to other proteins To examine whether the ice-induced perturbation observed for azurin is common to proteins in general, ANS binding experiments were extended to a set of seven other proteins including monomeric BSA, α-amylase and β-gal, dimeric LADH and tetrameric ALD, YADH, and LDH
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. MR images were acquired using a spin-echo sequence with simultaneous suppression of the lipid peaks between 0 and 3 ppm using three successive sinc3 pulses of 3 ms length and 2070 Hz bandwidth centered at 1
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. 12 The R-type flux is nonactivated rosin flux with only pure colophony in a proper solvent
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. 7 B); T-jumps to other temperatures show a similar trend
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. Each emerges from the high-dimensional hypergutter picture when a different projection into a low-dimensional space is applied
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. The simulations incorporate peak broadening due to interconversion of the proposed extended undocked and quasi-docked conformations (Fig
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. ProcedurePermission from the human subjects review board was granted to conduct the current study
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. There are seven Ospreys attached to HX-21, all MV-22Bs
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. The intensity changes of the various fluorophores upon heating could be classified into three distinct stages
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. Samples of EI^sup sc^ at a final concentration of 44
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. In EI^sup sc^ at low pH, the fluorescence intensity of ANS was largely enhanced, and the maximum wavelength of the fluorescence spectrum was 480 nm (Fig
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. As for azurin (12), lowering the freezing temperature from -5°C to -13°C increases the number of binding sites but the average affinity is lowered
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