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Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein
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The attempts to avoid artifacts in the 2D IR plots have been described in detail elsewhere (30)
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Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification
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Further localized MRS studies probing membrane permeability of drugs should thus seek to calibrate the individual intracellular spectra against data obtained in model membrane systems with known macromolecule concentrations
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They are clustered in the CaM:SEF2-1mp binding interface, the CaM:CaM dimer interface, and in the flexible helix connecting the CaM N- and C-terminal domains, and have similar exchange times (~50 µs), suggesting a cooperative mechanism probably caused by protein:protein interactions
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Once protein unfolding reached completion in the first jump, the sample was cooled and incubated at 25°C for 1000 s, which was sufficient for completion of the helical refolding (as indicated by flattening of the Θ^sub 222^(t)) but not for the disk reconstitution (as indicated by DSC, EM, and gel electrophoresis)
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On the Argentine continental shelf this family is represented by two species, Pseudopercis semifasciata (Cuvier, 1829) and Pinguipes brasilianus Cuvier, 1829
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There was no evidence of mental status changes, cerebrospinal fluid (CSF) rhinorrhea, or epistaxis
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We thank Sean Burnett, Wendy Eyres, and Suzanne Solven of the BC Ministry of Health for assistance with the collection and interpretation of the data
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The book consists of a very brief one-page introduction which sets out his aim to reach both the medical professionals and the educated public by avoiding unnecessary technical jargon without however sacrificing "scientific rigor, so that those who possess a suitable scientific background will be helped to take their first steps, perhaps, towards a science that is in need of radical renewal" (p
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