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In this research, the sequential events in ACYI thermal unfolding were investigated by a combination of spectroscopic methods and the related resolution-enhancing techniques
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Polymer principles in protein structure and stability
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Structural and dynamical properties of a denatured protein
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The mean residue ellipticity, [Θ], was obtained from the raw ellipticity data, Θ, as described (23), and all spectra were corrected with the proper baseline
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This different behavior in the thermograms among the members of a protein family is not new, and it has been reported, for instance, in the thermodynamic unfolding of dimeric histone-like proteins (53)
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Thermodynamic and folding properties versus structure among the members of a protein family Early studies suggested that folding and equilibrium pathways of homologous proteins were similar, and that the folding of a certain structure was thus conserved through evolution (58,59)
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In the presence of even slower internal motions it becomes progressively difficult to distinguish overall tumbling and internal motion (22,32)
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Below we consider equations assuming fast exchange
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Frenkel for helpful discussions and comments on the manuscript, and the Kavli Institute for Theoretical Physics for the hospitality of its program "Dynamics of Complex and Macromolecular Fluids", where the bulk of this work was done (preprint number NSF-ITP-02-55)
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