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In contrast to chaotrope-induced denaturation, thermal denaturalion of both rSBA and gSBA outlined a two-state process (Fig
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The error in the R^sub 1^ and R^sub 2^ values was defined as the standard deviation between three curve fittings, one with all time points and two fittings with reduced data sels containing every second time point, which were shifted either zero or one time point in the relaxation series
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Thirdly, the relaxation-compensated CPMG experiment at 600 MHz was repeated at a lower temperature (300 K instead of 308 K) to measure the change in ΔR^sub ex^, ΔΔR^sub ex^
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In contrast to reports on DNA binding with monocations, the compaction potentials of monocations determined here correlate well with biological assays that describe the compaction of chromosomes
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We repeated this study in a mild detergent, β-octylglucoside, since stronger detergents such as sodium dodecyl sulfate eliminate enzyme activity
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To compare the activation energy E^sub a^ of disk denaturation for different proteins, we recorded T-jump CD data of apoA-1:DMPC disks (Fig
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The behaviour of polyamino acids reveals an inverse side chain effect in amyloid structure formation
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This duplex has a 14-bp separation between donor and acceptor, and spFRET experiments show only a small change in E upon going from 0
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. In Flory's approach, a range of chain properties including R^sub g^, the average end-to-end distance (R^sub e^), the hydrodynamic radius (R^sub h^), the second virial coefficient (B^sub 2^), and the osmotic pressure (II) are calculated as series expansions in terms of the parameter z = N (T - Θ/T) (39,40,45)
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. MOHLENBROCK is professor emeritus of plant biology at Southern Illinois University in Carbondale
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