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As Abramoff, Buckham, and their associates built their influence empire, one of their most useful instruments was the nonprofit organization--groups like the U
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. The changes of thermodynamic parameters (enthalpy change ((Delta)H), entropy change ((Delta)S) and heat capacity change ((delta)C^sub p^)) corresponding to the transitions from N to MG (molten globule state) or MG to U (unfolded state), were summarized in the previous report (Nishii et al
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. Phase diagram for acidic conformational states of apomyoglobin
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. Intrinsic fluorescence spectroscopy The samples for the fluorescence measurements were prepared by dissolved ACYI in 30 mM Tris-HCl (pH 7
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. Über Spallungen und Synthesen im Thierkörper
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. Among them LDH, α-amylase, and β-gal have in the past been the subject of freeze-thawing inactivation studies (13-17)
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. The qualitative structural implication is, therefore, that the second CaM monomer in the dimeric complex is placed along this axis in such a way that the tips of the N- and C-terminal domains touch each other
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. The visible sample surface was then coated in silicone oil (200/500 cs) to prevent water loss
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. Only if the two sets of data showed accordance (occasionally an exact location of the apex is difficult), is the data further discussed
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. 2 shows the unheated insulin sample, which at room temperature (20°C) has little tendency to aggregate
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. Further investigations focused on the seasonal distribution of spawners are needed to confirm the existence of spawning aggregations indicated by the presence of larvae and posttransition juveniles
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. At each sampling time, the state of the system is queried, and the recorded photon is assigned to either donor or acceptor channel, with a probability based on the FRET efficiency of the detected system state
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. The MG concept was introduced to describe the properties of partially folded intermediate states sharing similar structural characteristics (i
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. It was difficult to distinguish in this research whether the pretransitional changes occurred in the catalytic domain or the dimerization domain of ACYI
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. Flory showed that a quantity such as the average radius of gyration (R^sub g^) will scale with chain length (N) according to a power law of the form R^sub g^ = R^sub o^N^sup ν^ (45)
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. Although P^sub oa^(r = 0) decreases linearly with cavity size, a, for the EV limit, it decays much more rapidly for the freely rotating chain version of ubiquilin
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. Third, these increased fluctuations could certainly lead to the occasional close approach of distal amino acids
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. The true number is probably larger because on average protein sites exhibit ANS fluorescence yields much lower than 0
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. Protein dynamics from NMR
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