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. In principle all these phenomena can alter the microenvironment of the intrinsic fluorophores and would therefore affect the emission spectra
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. The fact that for NDSB samples the obtained Δλ^sub max^-value between the folded and the unfolded state is much larger as compared to that measured with pure buffer samples is a further indication that aggregates reduce the magnitude of red shifts
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. Our experiments are consistent with soman-induced stabilization resulting from an elevation of the free energy of the unfolded state, which arises mainly from a decrease in its entropy due to the covalent modification by soman
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.Structural interpretation of the nanosecond-timescale internal motionThe dimeric CaM:SEF2-1mp complex can in principle show internal modes of motion involving either the individual N- and C- terminal domains and/or the CaM monomers as a whole
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. Therefore, the difference between DCVJ and ThT is that ThT does not fluoresce in the presence of protofibrillar amyloid of TTR at pH 3
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. Myoglobin forms amyloid fibrils by association of unfolded polypeptide segments
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