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. The pronounced blue shift for native proteins in NDSB solutions is most probably related to the fact that NDSB interacts with the surface of the proteins which causes a more hydrophobic nonpolar environment of surface exposed tryptophan residues
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. Size distribution functions were computed using the appropriate Windows-based ALV software, which incorporated regularized inverse Laplace transform and ALV-CONTIN packages
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. The fluorescence from ANS and Bis-ANS fluorophores show strict environmental dependence and are essentially nonfluorescent in water but are highly fluorescent in hydrophobic environments
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. For simplicity we assume that the kinetics of each diffusive search is single exponential with characteristic time τ
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. Nevertheless, the stabilizing effect of complexation on backbone and side chains can be unambiguously identified when comparing the buildup curves of the two states (Fig
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. In terms of frequency, there were differences in the immunostaining with TGF-[alpha] in both reactive mesothelial proliferations and mesotheliomas, but these differences did not achieve a level of significance (P =
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. And to make matters worse, according to OFHEO, when regulators began to catch on to what was happening, Raines and his team then "sought to interfere" with the OFHEO investigation by trying to get Congress to start up a separate probe of OFHEO
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.DISCUSSIONMolten globule (MG) folding intermediates have been characterized for a number of proteins (for reviews, see Kuwajima, 1989; Ptitsyn, 1992, 1995)
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. The spectra were measured after the samples had been equilibrated for 2 min at given temperatures controlled with a circulating water bath
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. The nonglycosylated form features a lower stability when compared to the glycosylated form
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. Although gSBA unfolded via a two-state process without the formation of any unfolding intermedialcs, the denaturation profile of rSBA was poputated with an intermediate (Fig
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. The ability to simulate denatured-state ensembles is important for a range of applications including protein design (25,26)
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. The contribution of vibrational entropy to molecular association
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. In contrast to the glasses and networks, these are relatively low viscosity liquid states
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. 1 illustrates DNA compaction in PEG 3000 with an increase in the NaCl concentration
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. 4, we show that Gα^sub s^ is destabilized under pressure even at relatively high lipid concentrations, although we note that membrane binding of Gα^sub s^ is weaker than Gβγ (i
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. 6 a shows a representative example of the morphological variability in insulin fibrillar aggregates grown for 10 min at 70°C
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. Nonetheless, it underlines the importance for overall folding rates of free-energy structures along a folding pathway, in which successive fluctuations in entropy and energy create a sequence of intermediate states
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.We conducted the subsequent tests for the effects of other factors on particle size using Isopar G, a dissolving temperature of 130°C, and a dissolving time of 2 hours
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. We thank Sean Burnett, Wendy Eyres, and Suzanne Solven of the BC Ministry of Health for assistance with the collection and interpretation of the data
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.* The National Council on Problem Gambling (NCPG) reports that 50 percent of teens who gamble are likely to binge drink and 75 percent smoke marijuana
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. Journal of Personality Assessment, 55, 630-639
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