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. Mechanisms of tryptophan fluorescence shifts in proteins
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. The authors thank Jacques Colletier for useful discussions on cholinesterase biochemistry as well as the IN16 instrumental team (Bernhard Frick, Thilo Seydel, and Matthias Elender) and the IN13-CRG team (Marc Bée, Francesca Natali, and Sebastien Vial) at the Institute Laue-Langevin, Grenoble, France
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. In the classic amyloidoses, accumulation of extracellular fibrillar aggregates called amyloid has been detected in various tissues depending on disease (Buxbaum, 2003)
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. From the fit it was obvious for all probes that there were burst phase responses going from the signal from the monomeric unfolded state (U) to the A-state
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. Time resolved fluorescence anisotropy of extrinsic probes as a measure of protein aggregationFluorescence lifetime data from ANS, Bis-ANS, DCVJ, and ThT were recorded from the probes bound to both native and aggregated TTR (Fig
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. Like De Cabo (3) we found that the first cranial bones that appeared during larval development in P
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. (11) used small-angle x-ray scattering (SAXS) to measure R^sub g^ as a function of N for 28 different chemically denatured proteins, with chain lengths varying from 8 to 549 residues
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. ^sup 15^N-{^sup 1^H} NOE values were determined from the ratios of peak volumes recorded in presence and absence of proton saturation
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.42 s^sup -1^ is obtained, which is very close to the average experimental R^sub 1^
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