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The results in Fig
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. Computational simulation of the statistical properties of unfolded proteins
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. COM/PH: 312-932-0400RELATED ARTICLE: Understanding milk
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. In addition, all thermal transitions in NDSB solutions exhibit also the missing reversibility as observed for pure buffer solutions
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. ), and by the National Creative Research Initiative (grant to B
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. The minimum torque limit was 2 × 10^sup -6^ Nm
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. Cross-linking of samples after incubation times >2 h showed a protein band that was unable to enter the gel, indicating formation of very large aggregates >1000 kD (data not shown)
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. The RNA strands were deprolected, purified by denaturing urea gel elcctrophoresis, desalted and subsequently purified by reversed-phase high-performance liquid chromatography utilizing an eluent system of acetonitrile and aqueous triethylammonium acetate (100 mM, pH 7)
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. t] equals 1 in November 1995 and thereafter (the Type 2 RP policy period), and equals 0 otherwise
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. Third, a slightly brown colored aqueous layer was dialyzed against water until the pH of the protein solution became 5
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. Since no high-resolution structure of ACYI is available, all of the Trp fluorophores (Classes A and S, I, II, and III) defined previously (34) were included in the fitting procedure
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. 1 A, open squares)', 2), the ANS-binding experiments (Fig
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. A change in the conformation of prions accompanies the emergence of a new prion strain
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