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3) of the spectra (29-31) were used for analysis of the percentages of secondary structure
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Taken together, these data suggest that there were structural changes, which partially solvent-exposed the indole moiety and/or tyrosine residues, and modified the ellipticity at 222 nm (41,42)
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The ANS binding test extended to other proteins confirmed a widespread stressed condition of the globular structure in ice, indicating that oligomers' proteins are more susceptible to unfolding than monomers', presumably because the macromolecule can be further destabilized by subunit dissociation
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When comparing ThT and DCVJ fluorescence data from a protofibrillar sample of TTR (24 h incubation), it is evident that DCVJ shows pHindependent fluorescence (Fig
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These enzymes catalyze the hydrolysis of a minor component in membranes, PI(4,5)P^sub 2^, to generate two second messengers which lead to a rise in intracellular calcium and activation of protein kinase C (see Rebecchi and Pentyala, 2000; Rhee, 2001)
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First, in contrast to lipid-free apoA-2, apoA-2 on DMPC disks shows no cold denaturation
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The first peak is centered at 28°C and reflects DMPC phase transition in intact disks that is not detected by CD (Fig
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