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2 mg/ml) similar to that used for the activity assay, except that the transitions occurred at a temperature ~2°C higher than the 1 mg/ml sample
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To understand bioactives, it is best to go to the model nature devised to deliver bioactives: milk
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However, our data indicate that aggregation and in addition at least one of these other mechanisms seems to play a role for the α-amylases with red shifts upon thermal unfolding (i
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For this test the samples were rapidly frozen in liquid nitrogen and then thawed at ambient temperature
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Spatial localization in NMR spectroscopy in vivo
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Liquid-liquid phase separation was observed at pH 5
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