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Secondary structure of proteins from circular dichroism spectra
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The DSC curves were obtained using a scanning rate of 0
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In other words, chains in the EV limit are not hard, prolate ellipsoids
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The stability of these mutants is ~3 kcal/mole lower relatively to the WT (20-22), and the internal flexibility of the polypeptide is considerably larger, particularly with I7S and F110S (18)
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Circular dichroism experiments were carried out on the DmAChE sample before and after the neutron scattering experiment, to see if any conformational change had occurred
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The ionic strength dependence of the solid-liquid coexistence line for BLG at pH 5
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11 B), amyloid fibrils of insulin, and lysozyme (P
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, 1999) to CPM-PLCβ gave a volume change similar to Gβγ-subunits (Table 1), which suggests that the contacts made by this peptide reflect the same ones made by the whole Gβγ-subunit (see Discussion)
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, 1986; Plager and Nelsestuen, 1992); however, some general guidelines have emerged
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Apolipoprotein A-II modulates the binding and selective lipid uptake of reconstituted high density lipoprotein by scavenger receptor BI
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Larvae were present in only 3
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, Personal communication, Sawgrass Technologies, Inc
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A complementary approach is to characterize the conformational distributions of freely diffusing RNA molecules using a single-molecule method referred to as diffusion spFRET (Deniz et al
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MATERIALS AND METHODSRNA constructs2'-0-bis(2-acetoxyethoxy)methyl orthoester (2'-ACE) protected RNA strands were purchased from Dharmacon (Boulder, CO)
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