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. Little information can be extracted by comparing the entropy of the two states
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. Thanks to an unusually blue shifted Trp fluorescence spectrum of native azurin, a measure of the protein fraction remaining native after freezing-thawing can be obtained directly from the residual fluorescence intensity at 300 nm (λ^sub ex^ = 292 nm), a wavelength where denatured-aggregated proteins were found to contribute little to the overall intensity
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